Determination of plant hormones in fertilizers by high-performance liquid chromatography with photodiode array detection: method development and single-laboratory validation.
نویسندگان
چکیده
A simple and reliable high-performance liquid chromatographic method that uses photodiode array detection was developed for the simultaneous determination of 12 native and synthetic plant hormones, i.e., plant growth regulators (PGRs), in fertilizers, such as 1-naphthol, 2,4-dichlorophenoxyacetic acid, 4-(2,4-dichlorophenoxy)butyric acid, 4-chlorophenoxyacetic acid, indole-3-acetic acid, 4-(3-indolyl)butyric acid, dichlorprop, (4-chloro-2-methylphenoxy)acetic acid, alpha-naphthaleneacetic acid, 1-naphthaleneacetamide, beta-naphthoxyacetic acid, and thidiazuron. The method was experimentally validated for routine regulatory application, and the following analytical parameters were assessed for all PGRs studied: linearity; specificity; precision (relative standard deviation) and accuracy, both measured at 3 concentration levels (0.1, 0.05, and 0.01%, w/w); ruggedness; limit of detection; and limit of quantification. Results were satisfactory for all method validation parameters tested and for all PGRs studied, demonstrating the suitability of the method for the determination of PGRs in fertilizers. The uncertainty of measurement was also estimated at 3 concentration levels for all PGRs by using the approach of the International Organization for Standardization, described in its Guide to the Expression of Uncertainty in Measurement. The method was applied to 20 samples of liquid fertilizer with declared biostimulant properties.
منابع مشابه
Development and validation of a HPLC-UV method for determination of Proscillaridin A in Drimia maritima
Background and objectives:Drimia maritima (L.) Stearn also known as squill is a medicinally important plant that has been used for various ailments such as dropsy, respiratory disorders, jaundice and epilepsy from ancient times. Proscillaridin A is identified as one of the most effective compounds in the plant with remarkable pharmacological features including efficacy...
متن کاملExtraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method
Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 µL of carbon tetrachloride (extraction solvent) was ...
متن کاملExtraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method
Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 µL of carbon tetrachloride (extraction solvent) was ...
متن کاملInterference-free Determination of Carbamazepine in Human Serum Using High Performance Liquid Chromatography: A Comprehensive Research with Three-way Calibration Methods
In the present study, a comprehensive and systematic strategy was described to evaluate the performance of several three-way calibration methods on a bio-analytical problem. Parallel factor analysis (PARAFAC), alternating trilinear decomposition (ATLD), self-weighted alternating trilinear decomposition (SWATLD), alternating penalty trilinear decomposition (APTLD) and unfolded partial least squa...
متن کاملInterference-free Determination of Carbamazepine in Human Serum Using High Performance Liquid Chromatography: A Comprehensive Research with Three-way Calibration Methods
In the present study, a comprehensive and systematic strategy was described to evaluate the performance of several three-way calibration methods on a bio-analytical problem. Parallel factor analysis (PARAFAC), alternating trilinear decomposition (ATLD), self-weighted alternating trilinear decomposition (SWATLD), alternating penalty trilinear decomposition (APTLD) and unfolded partial least squa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of AOAC International
دوره 91 6 شماره
صفحات -
تاریخ انتشار 2008